Whole Plasmid Sequencing Service
Obtain accurate and reliable full length plasmid sequencing with our Next Generation Sequencing service.
Specifications
| Reagent | jetPRIME® |
|---|---|
| Molecule delivered | DNA, siRNA, DNA & siRNA |
| Applications | Plasmid transfection |
| Cell types | Adherent cells |
| Number of transfections | 1.5 ml of jetPRIME® transfection reagent is sufficient to perform up to 1500 transfections in 24-well plates or 375 transfections in 6-well plates |
| Storage | Store jetPRIME® at 5 °C ± 3°C. |
| Provided with | jetPRIME® buffer |
Overview
jetPRIME® is a polyvalent transfection reagent that ensures effective and reproducible DNA and siRNA transfection in several adherent cell lines. This versatile reagent is also perfect for co-transfection of DNA and siRNA. Transfection with jetPRIME® requires only low amounts of nucleic acid, hence resulting in very low cytotoxicity.
jetPRIME® is suited for different applications, such as plasmid transfection, gene silencing, co-transfection of different nucleic acids, and CRISPR/Cas9-mediated gene knock-out.
Ordering Information
| Reference Number | Amount of reagent | Amount of buffer |
|---|---|---|
| 101000027 | jetPRIME® 0.1 mL | 5 ml |
| 101000015 | jetPRIME® 0.75 mL | 60 ml |
| 101000046 | jetPRIME® 1.5 mL | 2 x 60 ml |
| 101000001 | jetPRIME® 5 x 1.5 mL | 10 x 60 ml |
| 201000003 | - | jetPRIME® buffer 60 ml |
| 114-01 | 0.1 ml | 5 ml |
| 114-07 | 0.75 ml | 60 ml |
| 114-15 | 1.5 ml | 2 x 60 ml |
| 114-75 | 5 x 1.5 ml | 10 x 60 ml |
| 712-60 | x | 60mL |
One reagent for DNA and/or siRNA transfection
jetPRIME® is a polyvalent transfection reagent well suited for DNA and siRNA delivery. jetPRIME® is also ideal for DNA/siRNA co-transfection, ensuring high DNA Transfection and efficient gene silencing in a variety of cell lines with very low toxicity.
High transfection efficiency in a wide variety of adherent cell lines
jetPRIME® is an easy-to-use transfection reagent for day-to-day experiments that leads to efficient and reliable scientific results. It leads to outstanding high percentage of transfected adherent cell lines of various origins as well as primary cells.
Extremely gentle on cells
Transfection with jetPRIME® requires low amounts of reagent and nucleic acids, leading to increased cell viability while keeping high transfection efficiency.
Requires less DNA and less reagent
Transfection with jetPRIME® requires a small amount of reagent and DNA/siRNA, making its use very cost-effective
All Data
Other sources to help you understand jetPRIME® better
Other Sources
Check our FAQs
These FAQs are organized by application to guide you to find the best answer possible.
Product Documents
You have access to all the documents related to the transfection reagent.
Polyplus Database
Search for publications in our Transfection Database with Polyplus transfection reagents
Lexicon
This lexicon will help you to understand the different terms related to Polyplus-transfection®.
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Testimonials
KS
Kimberley S.
Royal Veterinary College, UK
"jetPRIME gave better transfection efficiencies than other reagents tested and much less cell death was noticed. The cells don't seem to mind being treated with the transfection reagent, there is no need to change the media anyway. I've already ordered a bigger volume of jetPRIME and have used half of it already! Will possibly be ordering some more soon!"
CR
Cosima R.
University Hospital Erlangen, DE
"I already ordered jetPRIME. It was very efficient and easy to use! Also the cells were in a good state after transfection. Good product!"
SH
Sean H.
MD Anderson Cancer Center, USA
"Very good transfection efficiency, better value and ease of use"
VA
Vijayalakshmi A.
Baylor College of Medicine (BCM), USA
"jetPRIME has low toxicity and with less amount of DNA and the transfection reagent comparable expression can be achieved. I would prefer using jetPRIME over other reagents for the above mentioned reasons."
MJ
Marlene J.
Temple University, USA
"Switching to jetPrime significantly impacted the success of our studies. In comparison to a competitors transfection reagent, jetPrime gives higher transfection efficiency, less toxicity and requires less plasmid DNA.
Thank you PolyPlus for developing this reagent for researchers."
JB
Jeetayu B.
Albert Einstein College of Medicine, USA
" We're still in love with the jetPRIME transfection reagent. It was really the only reagent that was satisfactory for our immortalized primary MEFs and allowed two projects in our lab to move forward much more quickly than our originally planned lentiviral strategy. Bravo!"
CH
Corinne H.
Ohio State University, USA
"I was so pleasantly surprised by the efficiency of jetPRIME reagent I ordered some the next day. MCF7 cells are notoriously hard to transfect and they are our primary cell line in the lab so I am so thankful to have this reagent at a reasonable cost."
EA
Esra A.
University of Sheffield, UK
"Many thanks for the jetPRIME sample I have received, I am more than happy and appreciate your outstanding service. I recommended the reagent to many researchers and students in the department they will order some very soon."
DN
Diogo N.
CNRS Bordeaux, France
"I'm very satisfied with jetPRIME. Good co-transfection efficiency with very low toxicity. One of the best I have ever tried."
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