Whole Plasmid Sequencing Service
Obtain accurate and reliable full length plasmid sequencing with our Next Generation Sequencing service.
Specifications
| Reagent | jetOPTIMUS® DNA Transfection Reagent |
|---|---|
| Molecule delivered | Plasmid DNA |
| Applications | Transient and stable gene expression from plasmid DNA transfection |
| Cell types | Adherent cells and hard-to-transfect cells: |
| Number of transfections | 1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol |
| Storage | Store jetOPTIMUS® at 5 °C ± 3°C. |
| Provided with | jetOPTIMUS® Buffer |
Overview
jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells resulting in higher transfection efficiency, even in hard to transfect cells. Tested on various primary cells and cancer cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression with minimal impact on cell viability and morphology.
jetOPTIMUS® is highly efficient for DNA delivery for various applications, such as transient and stable gene expression, CRISPR genome editing using DNA approach, as well as virus production in small scale.
Superior gene expression in easy-to-hard-to-transfect cells
jetOPTIMUS® improves DNA transfection efficiency in easy- and difficult-to-transfect cells used as in vitro cell culture models. Tested on various primary cells and cancer cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression than main competitors.
High gene expression using low DNA amount
jetOPTIMUS® has been developed to increase transfection efficiency into a wide range of cell types, while keeping the amount of DNA as low as possible and reducing the volume of reagent required compared to main competitors.
Excellent cell viability and morphology
Reaching high transfection efficiencies is often at the expense of cellular integrity, with adverse effects on cell cycle, metabolism and signaling pathways. At Polyplus-transfection, we make no compromise on cell integrity: jetOPTIMUS® transfection reagent is gentle on cells, preserving cell viability and morphology, which allows generation of biologically relevant data from gene expression studies.
Time-saving with optimized ready-to-use protocol
jetOPTIMUS® is a ready-to-use transfection reagent provided with its own complexation buffer (jetOPTIMUS® Buffer). The protocol is optimized for simplicity of use (all plate sizes), culture medium compatibility (antibiotics, serum) and cost-effectiveness (lowest amount of DNA and volume of reagent).
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They used it
Testimonials
HP
Helena P, University of Lund , SE
"I have tested many different transfection reagents during the past 15 years and I have never been as impressed as I was today by the performance of jetOPTIMUS. Excellent viability at 24h and remarkable transfection efficiency (pEGFP-C1). The 100 µl free sample was generous and we will evaluate it further in our experiments during the coming month. If results continue to look this good we will definitely order the reagent"
GF
Gautier F, University of Turku, FI
Postdoctoral Researcher
"There is a massive improvement with the jetOPTIMUS® compared with gold standard Lipofectamine. Something like 30% more transfected cells, no mortality."
LM
Laurent M., Centre de Recherches en Cancérologie de Toulouse, FR
"Our lab recently started using jetOPTIMUS® for our transfection work as we were needing to improve transfection efficiency. We found that JetOPTIMUS was superior to other tested reagents in all of the cell lines we tested."
MJ
Marlene J., Temple University, US
"Where other methods have failed, jetOPTIMUS® works in transfecting primary fibroblast cultures with high transfection efficiency and low toxicity"
DK
Danai L., Katholieke Universiteit Leuven, BE
“In comparison to another transfection reagent, jetOPTIMUS® was mind-blowingly better for the difficult to transfect 3T3-NR6 cells!“
AU
Ari Uyar O., Gebze Technical University, TR
"Very easy to use, sensitive cell lines support it very well, good efficiency and reproducibility, I would recommend it."
OO
Ofek O., Ben Gurion University of the negev, IL
"I tried both jetOPTIMUS® and jetPRIME® transfection reagents on SH-SY5Y cells, both worked very well as I observed approximately 90% transfection efficiency. I would recommend it."
MS
Mona S., University, DE
“jetOPTIMUS® is a great product. It worked better than competitors to deliver large plasmid constructs into hard-to-transfect cells."
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