Protein Manufacturing Service
Our fully integrated protein manufacturing services covers all needs from initial process development to manufacturing at research, HQ and GMP grade.
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Specifications
| Reagent | FectoPRO® |
|---|---|
| Molecule delivered | DNA |
| Applications | Protein and mAb production |
| Cell types | Suspension CHO, HEK-293, and derivatives |
| Number of transfections | 1 mL of FectoPRO® is sufficient to transfect 800 ml to more than 1 liter of cell culture |
| Storage | Store FectoPRO® at 5 °C ± 3°C. |
| Provided with | FectoPRO® Booster |
Overview
FectoPRO® Transfection reagent is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount (< 1 µg/ml of cell culture).
FectoPRO®-mediated transfection process is easily scalable from a few ml to several liters of cell culture, ensuring robust reproducible protein production. Increased productivity and low DNA amount requirement make FectoPRO® a cost-effective solution for bioproduction processes.
Ordering Information
| Reference Number | Transfection Reagent | Expression Booster | Expression Medium |
|---|---|---|---|
| 101000014 | FectoPRO® 1 mL | 1 mL | |
| 101000007 | FectoPRO® 10 mL | 10 mL | |
| 101000019 | FectoPRO® 100 mL | 100 mL | |
| 101000011 | FectoPRO® 1 mL | 1 mL | FectoCHO® CD Medium 1 |
| 101000003 | - | - | FectoCHO® CD Medium 6 x 1 L |
| 116-001 | 1 mL | 1 mL | ***NODISPLAY*** |
| 116-010 | 10 mL | 10 mL | ***NODISPLAY*** |
| 116-100 | 100 mL | 100 mL | ***NODISPLAY*** |
| 716-06L | - | - | 6 x 1 L |
| 716-01LKIT | 1 mL | 1 mL | 1 L |
Amazing protein and antibody production yields
FectoPRO® gives superior transfection efficiency leading to higher protein production yields in both suspension CHO and HEK-293 cells compared to other transfection reagents such as linear PEI
FectoPRO® is cost-effective
On top of the production yields improvement, FectoPRO® saves 40 to 60% on DNA cost by using 0.4 to 0.6 µg DNA / 10^6 cells / ml of cell culture; hence making this kit an affordable and economical solution for Bioproduction processes.
FectoPRO® is easily scalable
Thanks to its robust protocol easily adapted to various culture vessels, FectoPRO®-mediated transfection can be scaled linearly from 30 ml up to liters of cell culture without protocol adaptation making it a perfect solution to streamline your bioproduction process.
FectoPRO® is compatible with various commercially available expression systems using either HEK293 or CHO cells.
When used together with the FectoCHO® CD medium, FectoPRO® gives outstanding protein yields in combination with various CHO cell lines.
Find out more about the FectoCHO® Expression System
All Data
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Product Documents
You have access to all the documents related to the transfection reagent.
Polyplus Database
Search for publications in our Transfection Database with Polyplus transfection reagents
Lexicon
This lexicon will help you to understand the different terms related to Polyplus-transfection®.
Testimonials
CE
Cupo et al, 2019
"The solution we identified was to eliminate the need for the ExpiCHO-S cell Enhancer component by using instead a different transfection medium culture system, FectoPRO® with Booster, with the ExpiCHO-S cells. We also note that the FectoPRO® with Booster system is ~40% cheaper than its ExpiFectamine CHO with Feed and Enhancer counterpart. This methodology change would therefore also provide cost savings."
doi: 10.1371/journal.pone.0215106.
PF
Philippe F.
ProteoGenix SAS, France
"In our hands, FectoPRO® gives the highest protein production yields we ever reached using transient transfection. Our company offers custom services including protein production in mammalian cells. Being able to produce a lot more protein without revisiting our expression system opens a whole new range of possibilities for our antibody and protein production services."
TC
Thomas C.
Biotech, Cambridge, MA, United States
“I have to admit I had low expectations for FectoPRO®. Perhaps a modest improvement in transfection … an extra 5-10%. J. did her first experiment yesterday and analyzed it today and the result was remarkable. I’m shocked by both the efficiency of transfection and the brightness of the GFP.”
JL
James L.
Biotech company, United States
“FectoPRO® yields transient expression titers in HEK, matching or exceeding those from other suppliers, but at a fraction of the price.”
JJ
Jean-Philippe J.
Hospital for Sick Children, Canada
“2 x the yield for half the amount of transfection reagent = 4 x less cost. It is also very easy to use.”
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