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Specifications
| Reagent | jetMESSENGER® |
|---|---|
| Molecule delivered | mRNA |
| Applications | Gene expression |
| Cell types | Hard-to-transfect cells |
| Number of transfections | 1.5 ml of jetMESSENGER® transfection reagent is sufficient to perform up to 1500 transfections in 24-well plates or 375 transfections in 6-well plates |
| Storage | Store jetMESSENGER® at 5 °C ± 3°C. |
| Provided with | mRNA buffer |
Overview
jetMESSENGER® is a highly efficient and gentle mRNA transfection reagent. jetMESSENGER® has been specifically designed to offer outstanding transfection efficiency in cells that are usually difficult to transfect, such as primary cells, cancer cell lines, neurons, and stem cells. jetMESSENGER® can also be used on a wide variety of easy to transfect cells. Transfection with jetMESSENGER® leads to very low cytotoxicity as it requires low amounts of mRNA and low volumes of reagent.
jetMESSENGER® is perfectly suited for gene expression, CRISPR gene editing, iPS generation, stem cell differentiation and immunotherapy assays.
Outperforms DNA transfection
jetMESSENGER® is a highly efficient and gentle mRNA transfection reagent. mRNA transfection presents many advantages versus DNA transfection:
- no risk of genome integration, hence no genome modification of the transfected cell
- no promoter regulation issue
- no need to reach the nucleus for efficient expression
- more gentle process
Allows higher gene expression than main competitors on a variety of cell lines
jetMESSENGER® is extremely efficient especially in hard-to-transfect cells compared to other mRNA transfection reagents. Transfection with jetMESSENGER® leads to outstanding mRNA delivery in a wide variety of cell lines, such as neurons, primary cells, stem cells and various cancer cell lines,
Suitable for different applications
jetMESSENGER® is designed for several applications, such as gene expression, CRISPR/Cas-9 gene editing, reprogramming experiments in fibroblasts and stem cells, and immunotherapy assays.
Extremely gentle on cells
By eliminating the need to reach the nucleus for efficient expression, jetMESSENGER® allows transfection of quiescent and slow dividing cells. In addition, jetMESSENGER® operates through an extremely gentle process. Cell viability remains extremely high during transfection and cell morphology is maintained.
All Data
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Product Documents
You have access to all the documents related to the transfection reagent.
Polyplus Database
Search for publications in our Transfection Database with Polyplus transfection reagents
Lexicon
This lexicon will help you to understand the different terms related to Polyplus-transfection®.
Testimonials
They used it
NL
Nabil L
Pharmaceutical company, France.
"After some optimization done with the help of Polyplus-transfection Scientific Support, I obtained with jetMESSENGER® an excellent transfection efficiency while maintaining a good viability in primary NK cells and NK cell lines."
YN
Y. N, Vervetx
“We observed outstanding transfection efficiency with jetMESSENGER in our Human Adrenal Gland cells; over 80% gene editing where Messenger-MAX and Stem-MAX maxed out at 45-50%. Bravo!”
RC
Remi C.
Columbia University, New York, NY
“We were very happy with the performance of jetMESSENGER and in vivo-jetRNA in delivering our antigen-expressing mRNA to a variety of cells in vitro and in vivo. The response achieved in relation to dose exceeded our expectations, without any observable adverse effect from the formulation.”
IT
Itkin T.
Weill Cornell Medicine, New York, United States
“Application of jetMESSENGER reagent to introduce modified RNA molecules into primary lung mouse endothelial cells (a cell population which is generally hard-to-transfect with DNA/RNA using non-viral methodology) resulted with the highest transfection rate (>70%) and with the lowest toxicity effects as compared to other tested RNA/DNA transfection reagents (jetPEI, TransIT, & RNAiMAX).”
BE
Brotin E., Abeilard E.
Denoyelle C., Poulain L. Inserm U1199 « BioTICLA », Caen, France
“jetMESSENGER appears as a very effective reagent for RNA transfection in human cancer cells of various origins. The expression level intensity and the transfected cells proportion were very impressive as compared to conventional DNA transfection reagents, without any sign of cytotoxicity.”
HM
Herb M.
University of Cologne, Germany
“The protocol and handling of jetMESSENGER Kit was very simple and easy to use in comparison to other mRNA kits we tested. In our hands, jetMESSENGER is the only component that resulted in 60% transfection efficiency in peritoneal macrophages, in less than 9h while not affecting cell viability.”
PJ
Pollet J
Baylor College of Medicine (BCM), USA
“The jetMESSENGER kit makes mRNA transfections very easy. The one step protocol is easy to use and can be combined with media comprising serum. It is a great tool!”
SC
Stefano C
Baseclick GmbH, Germany
“The experiments I did were really good, the transfection worked greatly and I did also a comparison with another transfection kit from a competitor, I have to say yours is much better.”
KS
Kathleen S
Pharmaceutical company, Germany
“I obtained a very good transfection efficiency using jetMESSENGER®. It is easy to use and the protocol is well described."
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